Real-time signal processing
Acquire, filter, and analyze weak fluorescence peaks while droplets move rapidly through the detection region.
MICROFLUIDIC SORTING
Fluorescence-activated microfluidic droplet sorting
Laser-induced fluorescence, photomultiplier acquisition, FPGA signal processing, and high-voltage pulse control form an end-to-end path that directs selected droplets into the target channel.
- Application
- Fluorescent droplet selection
- Real-time core
- NI FPGA
- Control path
- PMT acquisition to high-voltage pulse
PROJECT CONTEXT
Detection, decision, and sorting in a microsecond-scale path
Drug-labelled cells fluoresce under laser illumination, and photomultiplier tubes feed the signals into the FPGA. Acquisition, filtering, waveform analysis, and selection decisions complete at microsecond scale.
When a droplet meets the selection condition, the FPGA generates a high-frequency pulse for the high-voltage generator. Dielectrophoresis then directs the target fluorescent droplet into its designated channel.
Engineering focus
Precise triggering
Align the sorting decision and high-voltage pulse within the correct timing window for the target droplet.
Cross-domain commissioning
Coordinate optics, microfluidics, electrical systems, high voltage, and software while accounting for fluid viscosity and other conditions that influence sorting.
Real-time sorting path
Every stage from fluorescence detection to droplet deflection is synchronized around timing consistency.
Laser excitation
Labelled cells pass through the detection region in droplets and emit identifiable fluorescence.
PMT acquisition
Photomultiplier tubes convert fluorescence into electrical signals for the real-time path.
FPGA analysis
Filtering, waveform analysis, and selection logic produce the corresponding control pulse.
Dielectrophoretic sorting
The high-voltage field deflects qualifying droplets into the designated channel.
Delivered value
Complete real-time loop
Connect signal acquisition, algorithmic decision, and actuation in one continuous sorting process.
Visual commissioning
Bring PMT waveforms, scatter plots, thresholds, and high-voltage state into one software interface.
Continued optimization
Iterate FPGA logic and critical parameters to improve filtering and selection behavior.
System in operation
Four clips show FPGA filtering, droplet selection, the microfluidic chip, and the software during operation.
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